School of Materials Seminar entitled "De novo engineering of solid-state heme proteins"
|Starts:||10:00 27 Jun 2018|
|Ends:||11:00 27 Jun 2018|
|What is it:||Seminar|
|Organiser:||School of Materials|
|Speaker:||Dr Trevor D. Rapson|
The ability to harness the functional properties of heme proteins within a solid-state material affords a number of commercial advantages such as stability and recyclability in heterogeneous biocatalysts and biosensors.
To produce solid-state heme proteins we employ a recombinant coiled coil silk protein from honeybees as a de novo engineering scaffold.
This recombinant protein can be produced at commercially viable levels, the protein sequence can be precisely manipulated using molecular biology, allowing very fine control over the properties of the heme-binding
sites, and the protein can be fabricated in a variety of solid-state material forms such as films and sponges. We introduce heme cofactors to the silk materials and they interact through specific binding and coordination
sites akin to naturally occurring proteins. We will outline heme protein materials we have developed for nitric oxide breath sensing, oxygen reduction electrocatalysts and hydrogen evolution.
1. T.D. Rapson et al. (2015). De novo engineering of solid-state metalloproteins using recombinant coiled-coil silk. ACS Biomaterials science and engineering. 11, 1114-1120.
2. T.D. Rapson et al. (2017) Bioinspired electrocatalysts for oxygen reduction. 5, 10236-10243.
3. T.D. Rapson et al. (2017) Design of silk protein with increase heme binding capacity. 177, 219-227.
4. M.M. Musameh et al. (2018) Silk provides a new avenue for third generation biosensors, 103, 26-31.
Trevor Rapson received his PhD from the University of Queensland where he used protein film voltammetry to study metalloenzymes. He joined CSIRO in 2012 as an OCE Postdoctoral Fellow and is currently
a Research Scientist in the Health and Biosecurity Business Unit. His research interests include sensor development, bio-inspired material design and protein electrochemistry.
Dr Trevor D. Rapson
Role: CSIRO, Black Mountain, Canberra
Travel and Contact Information
Manchester Interdisciplinary Biocentre - John Garside Building