Seminar - Lymphocytes: function and migration in complex environment
| Dates: | 11 September 2018 |
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| Times: | 12:00 - 13:00 |
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| What is it: | Seminar |
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| Organiser: | Faculty of Biology, Medicine and Health |
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| Who is it for: | University staff |
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| Speaker: | Anne Reversat |
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Leukocytes are immune cells that move through the whole organism for protection against pathogens. Although all mammalian cells require integrins to migrate on 2D, observations in 3D environments reveal that 2D overemphasizes adhesion and integrin-requirement. Here, by combining CRISPR-Cas9 gene editing, engineered PDMS-based microenvironments (such as pillar mazes and microchannels) and videomicroscopy, we show that in complex structures, leukocytes such as T lymphocytes move in an amoeboid mode independently of adhesions. These mechanical forces are produced by the actin cytoskeleton and depend of the environmental topographies, findings that support the notion of deformation-based force transduction in the absence of transmembrane force coupling. Finally, those observations explain how leukocyte fast motility is performed in vivo in complex 3D environments completely autonomously from the composition of the environment. How lymphocytes, such as B cells and their differentiated counterparts the antibody-producing cells, can couple their immune functions with their migratory capacity remains to be solved.
All welcome.
Speaker
Anne Reversat
Organisation: Institute of Science and Technology Austria, Klosterneuburg, Austria
Travel and Contact Information
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1.006
AV Hill Building
Manchester
