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PRODID:-//Columba Systems Ltd//NONSGML CPNG/SpringViewer/ICal Output/3.3-
 M3//EN
VERSION:2.0
CALSCALE:GREGORIAN
METHOD:PUBLISH
BEGIN:VEVENT
DTSTAMP:20150527T132545Z
DTSTART:20150622T110000Z
DTEND:20150622T120000Z
SUMMARY:Systems Biology of the Extracellular Niche in Chronic Lung Diseas
 e
UID:{http://www.columbasystems.com/customers/uom/gpp/eventid/}m4d-ia6sblt
 t-bsqq33
DESCRIPTION:This is a FLS Special Seminar. \n\nAbstract: Despite the trem
 endous interest in ECM biology in the context of regenerative medicine a
 nd cancer research\, the proteomic characterization of extracellular mat
 rix niches is a much underexplored area of research. We develop advanced
  mass spectrometry workflows to study cellular and extracellular composi
 tion of tissues and the signalling landscape of cellular mechanosensing 
 (shotgun proteomics)\, as well as the architecture of ECM structures (cr
 osslinking mass spectrometry)\, and currently apply them to the process 
 of fibrosis and regeneration in the lung. \nIn the first part of my semi
 nar\, I will give an overview of the range of mass spec workflows we are
  using and possible future developments and research directions. In the 
 second part of my seminar I will highlight our recent study on lung inju
 ry and repair\, which was just accepted for publication in EMBO Molecula
 r Systems Biology. \n\nIn this study\, we monitored remodeling of the ex
 tracellular niche in tissue repair in the bleomycin-induced lung injury 
 mouse model. Mass spectrometry quantified 8366 proteins from total tissu
 e and bronchoalveolar lavage fluid over the course of eight weeks\, surv
 eying tissue composition from the onset of inflammation and fibrosis to 
 its full recovery. Combined analysis of proteome\, secretome and transcr
 iptome highlighted post-transcriptional events during tissue fibrogenesi
 s and defined the composition of airway epithelial lining fluid. To comp
 rehensively characterize the ECM\, we developed a quantitative detergent
  solubility profiling method (QDSP)\, which identified Emilin-2 and Coll
 agen-XXVIII as novel constituents of the provisional repair matrix. QDSP
  revealed which secreted proteins interact with the ECM\, and showed dra
 stically altered association of morphogens to the insoluble matrix upon 
 injury. Thus\, our proteomic systems biology study assigned proteins to 
 tissue compartments and uncovered their dynamic regulation upon lung inj
 ury and repair\, potentially contributing to the development of anti-fib
 rotic strategies. 
STATUS:TENTATIVE
TRANSP:TRANSPARENT
CLASS:PUBLIC
LOCATION:Lecture Theatre\, Michael Smith Building\, Manchester
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