Polarity reversal during EMT
| Dates: | 9 February 2016 |
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| Times: | 13:00 - 14:00 |
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| What is it: | Seminar |
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| Organiser: | Faculty of Life Sciences |
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| Who is it for: | University staff, Current University students |
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| Speaker: | Dr Manuel Thery |
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During epithelial to mesenchymal transition (EMT), cells lining tissue periphery break up their cohesion to migrate within the tissue. This dramatic reorganization involves a poorly characterized remodeling of the baso-apical polarity of static epithelial cells into the front-rear polarity of migrating mesenchymal cells. We monitored centrosome position during this transition in developing mouse embryos and organs, in cultured 3D cell aggregates and in micro-patterned cell doublets. In all conditions, centrosome left their peripheral localization next to cell-cell junctions to reposition at the cell center, while nuclei were moved away from cell-matrix adhesions. This resulted in an effective complete cell polarity reversal, accompanied by a redistribution of intracellular tensional forces. Sequential release of cell confinement using dynamic micropatterns showed that polarity reversal promotes cells disengagement and scattering. These results revealed that polarity was not loss during EMT but on the opposite that cytoskeletal remodeling and polarity reorientation were highly controlled and coordinated to direct cell scattering across the basement membrane.
Speaker
Dr Manuel Thery
Organisation: CEA, Grenoble
Travel and Contact Information
Find event
Michael Smith Lecture Theatre
Michael Smith Building
Manchester
