IBIC webinar series
| Dates: | 23 January 2025 |
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| Times: | 12:00 - 12:50 |
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| What is it: | Webinar |
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| Organiser: | Industrial Biotechnology Innovation Catalyst |
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| Who is it for: | Early years, University staff, External researchers, Alumni, Current University students, General public |
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A 'Lunch and Learn' webinar for anyone interested in industrial biotechnology brought to you by the Industrial Biotechnology Innovation Catalyst (IBIC)
January's webinar will be titled "Repurposing serine integrases as tools for engineering biology"
Presented by Femi Olorunniji and Alexandria Holland from Liverpool John Moores University
Summary: A key aspect of genome engineering is the ability to modularly construct and edit genetic circuits, to precisely programme selective gene expression and to manipulate multiple sets of genes that direct cellular structure and function. To build such programmable genetic circuits, we are developing Large Serine Integrases (LSIs) as orthogonally-acting tools that mediate predictable, controllable, and reversible rearrangements of DNA modules. LSIs have been widely used to promote the insertion of foreign DNA into the genomes of cells, plants and animals. New structural and biochemical insights into LSI-RDF interactions can be applied to develop more flexible engineering biology tools. Finding RDFs by sequence alone has been a bottleneck in the field. We found that AlphaFold2-multimer can be used to perform “virtual pulldowns” to identify putative RDFs. Wet lab testing of these predictions shows a high rate of true positives.
Register with the Registration form link on the right
Travel and Contact Information
