Sam Daly -- Advances in volumetric super-resolution microscopy and single particle tracking [IN PERSON]
| Dates: | 31 March 2025 |
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| Times: | 14:00 - 15:00 |
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| What is it: | Seminar |
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| Organiser: | Department of Mathematics |
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| Who is it for: | University staff, External researchers, Current University students |
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| Speaker: | Sam Daly |
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Join us for this seminar by Sam Daly (Cambridge) as part of the North West Seminar Series in Mathematical Biology and Data Sciences. More details about the joint series can be found here https://northwestseminars.great-site.net/ .
The talk will be hosted via zoom, please contact carl.whitfield@manchester.ac.uk or igor.chernyavsky@manchester.ac.uk for the zoom link, or sign up to the mailing list.
Title: Advances in volumetric super-resolution microscopy and single particle tracking
Abstract: Single-molecule localisation microscopy (SMLM) has enabled optical microscopy to probe biological structures and processes at the nanometer scale. To accommodate the three-dimensional nature of biology, 3D-SMLM techniques—such as the astigmatic and double-helix point spread functions (PSFs)—have been developed. These methods are inherently slow, limiting biological throughput and compatible labelling strategies. Accelerating 3D-SMLM is essential for broader applicability in the life sciences. This talk focuses on single-molecule light field microscopy (SMLFM), a novel 3D-SMLM approach that incorporates a microlens array in the back focal plane of an optical microscope to capture fluorescence from multiple perspective views. SMLFM facilitates an order-of-magnitude improvement in imaging speed compared to the gold-standard double-helix PSF opening up 3D-SMLM to new questions of adaptive immunity and protein secretion in fixed and living cellular systems.
References
1 Sims et al. https://doi.org/10.1364/OPTICA.397172
2 Daly et al. https://doi.org/10.1038/s41467-024-45828-5
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Speaker
Sam Daly
Role: Research Associate
Organisation: University of Cambridge
Travel and Contact Information
Find event
2.61
Simon Building
Manchester
