Florien Pein - Model-Free Idealisation of Patchclamp Recordings
|Dates:||3 March 2021|
|Times:||13:00 - 14:00|
|What is it:||Seminar|
|Organiser:||Department of Mathematics|
|Who is it for:||University staff, External researchers, Current University students|
Join us for this seminar by Florien Pein (Cambridge) as part of the North West Seminar Series in Mathematical Biology and Data Sciences. Details of the full series can be found here https://www.cms.livjm.ac.uk/APMSeminar/
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Abstract: The voltage patchclamp technique is a fundamental tool for the quantitative analysis of electrophysiological processes of transmembrane proteins, in particular of ion channels. Analysis of patchclamp recordings is often a challenging issue. In this talk we present a very general non-parametric model for such recordings. Based on this model we provide fully automatic model-free idealisation methods or in statistical words non-parametric estimation methods. Patchclamp recordings are typically lowpass filtered to suppress high-frequent noise components. Hence, a major point of our approaches is to take the filtering into account explicitly.
In a second step, we show how idealisations allowing us to obtain biochemical relevant information about the underlying gating dynamics. Finally, we discuss advantages and disadvantages of model-free approaches in comparison to hidden Markov model approaches and explain how they complement each other. Our methods are available as an R-package and as a graphical user interface.
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Organisation: Cambridge University
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