Advances in Biosciences Seminar - Konstantinos Beis, Imperial College London. Title: The peculiarities of transporters
| Dates: | 23 April 2024 |
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| Times: | 13:00 - 14:00 |
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| What is it: | Seminar |
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| Organiser: | Faculty of Biology, Medicine and Health |
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| Who is it for: | University staff |
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| Speaker: | Konstantinos Beis |
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Abtract: Transporters are found in all forms of life, and they are associated with the import of nutrients or the export of antibiotics and xenobiotics causing multidrug resistance. Transporters can be classified either as ABC transporters (ATP-driven) or secondary active transporters (proton/sodium driven). I will present recent work from the lab on two transporters that display certain peculiarities compared to the accepted working models in the field. The antibacterial peptide transporter SbmA is involved in peptide transport in gram-negative bacteria; this is a rather unique transporter that has an ABC transporter like fold (we call it SbmA-like peptide transporter fold (SLiPT)), but it is energised by the proton gradient rather than ATP. Mechanistically, SbmA behaves like an ABC transporter from our cryo-EM structures and functional data. The second oddity is the multidrug resistance protein 2 (MRP2); MRP2 exports bilirubin and it is associated with chemotherapy resistance in the liver canalicular membrane. The activity and function of MRP2 is regulated by phosphorylation and drug-drug interactions. Our cryo-EM structures, phosphoproteomic analysis and transport data in human hepatocyte-like cells have allowed us to gain mechanistic insights on this rather peculiar regulation by both endogenous kinases and drugs.
Speaker
Konstantinos Beis
Role: Reader
Organisation: Imperial College London
Biography: PhD at the University of St Andrews under the supervision of Prof James Naismith Postdoc at the Scripps Research Institute under the supervision of Prof Ian Wilson RCUK Fellowship, Senior Lecturer and Reader at Imperial College London
Travel and Contact Information
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Michael Smith Lecture Theatre
Michael Smith Building
Manchester
