Wnt proteins in asymmetric stem cell division and tissue engineering
|Starts:||14:00 20 Nov 2015|
|Ends:||15:00 20 Nov 2015|
|What is it:||Seminar|
|Organiser:||Faculty of Life Sciences|
|Who is it for:||University staff, Current University students|
|Speaker:||Dr Shukry Habib|
Part of the Manchester Tissue Regeneration & Stem Cell Network seminar series. Sponsored by PCR Biosystems.
Developmental signals such as Wnts are often presented to cells in an oriented manner. To examine the consequences of local Wnt signalling, we immobilised Wnt proteins on beads and introduced them to embryonic stem cells in culture. At the single-cell level, the Wnt-bead induced asymmetric distribution of Wnt-?-catenin signalling components, oriented the plane of mitotic division, and directed asymmetric inheritance of centrosomes. Before cytokinesis was completed, the Wnt-proximal daughter cell expressed high levels of nuclear ?-catenin and pluripotency genes, whereas the distal daughter cell acquired hallmarks of differentiation. We suggest that a spatially restricted Wnt signal induces an oriented cell division that generates distinct cell fates at predictable positions relative to the Wnt source. Based on the ability of localised Wnt proteins to instruct cell fate, my lab has recently developed a new Wnt platform with easy storage and long lasting activity. We demonstrated that this platform is able to maintain adult and embryonic stem cells while also being adaptable for 3D tissue engineering.
Dr Shukry Habib
Organisation: King's College London
Travel and Contact Information
Michael Smith Lecture Theatre
Michael Smith Building